Laser Scanning Confocal Microscope Magnification

Fluorescent microscopy not only makes our images look good it also allows us to gain a better understanding of cells structures and tissue.

Laser scanning confocal microscope magnification.

Laser scanning confocal microscopy laser scanning confocal microscopes employ a pair of pinhole apertures to limit the specimen focal plane to a confined volume approximately a micron in size. In the past the traditional laser microscope excited the whole thickness of the sample resulting in saturated blurry images and sometimes visualizing false colocalization images. With confocal laser scanning microscopy clsm we can find out even more. Capturing multiple two dimensional images at different depths in a sample enables the.

The confocal laser scanning microscope s aim was not to further increase magnification but to make clearer. Clsm combines high resolution optical imaging with depth selectivity which allows us to do optical sectioning. This means that we can view visual sections of tiny structures that. The laser scans across the object and an image is built up pixel by pixel on a screen.

The confocal laser scanning microscope clsm is a microscope which focuses only on a single focal plane and the unfocused plane will not be visualized.