When applied to plant cells and especially to woody tissues lcm requires extensive optimization to overcome such factors as rigid cell walls large central vacuoles intercellular spaces and technical issues with thickness and flatness of the sections. Medicina article validation of laser capture microdissection protocol in endometriosis studies katiane de almeida da costa 1 helena malvezzi 1 bruno gallani viana 1 renée zon filippi 1 rosa maria neme 1 2 thiago pinheiro arrais aloia 1 sérgio podgaec 1 and carla de azevedo piccinato 1 1 hospital israelita albert einstein são paulo br 05652 900 brazil 2 centro de endometriose são. At the moment we are using a protocol with 24h formalin fixation and 24h decalcification but the achieved rna quality after laser capture microdissection is poor and also immunofluorescence.
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